FrameStar® 96 Well Skirted PCR Plate
96 well skirted plates designed for use on standard thermal cyclers. Low profile wells, recommended for reaction volumes <20µl. Rigid frame eliminates warping and distortion during PCR. Ideal for use with robotic systems.
- PP wells, PC frame; cut corner H1
- <100µl working volume, 200µl total well capacity
- Available barcoded on request
- Ultra-low DNA binding option available
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*Online availability to US only
Product Details
Key Features
Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Stackable
Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
- Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
- Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Recommended for low volume PCR
- Ideal for use with robotic systems
- Compatible with standard multichannel pipettes
Options
- Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, black, and white
- Also available with a black frame and with a clear frame for the white well variety, for optimum signal-to-noise ratio when using fluorescent based assays
- Also available as a black frame with black wells for non-PCR fluorescent applications
- Extra rigid skirt option (4ti-0960/RIG) for use with Perkin-Elmer®Sciclone, Beckman, Hamilton, and other automation systems: eliminates the robotic grip picking up more than one plate at a time
- Ethylene oxide treated option available (4ti-OX960C/SBC) for forensic use; learn more about our ethylene oxide treated range
- Ultra-low DNA binding option available (4ti-LB0960/RIG) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
- Available barcoded upon request; learn more about our barcoding options
- Clear Polystyrene Lid (4ti-0287) available for 4ti-0960/RIG
Low DNA Binding Plates
- Azenta Life Sciences offer low binding products, recommended to support sensitive techniques such as qPCR and Next Generation Sequencing (NGS), as well as storage of samples.
- Manufactured using selected low-bind polymers, DNA loss is minimized during incubation and sample transfer, making it the ideal solution for use in applications with low DNA input.
- Low binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics. No contamination or modification of the samples.
- Tested under a broad temperature range. Maximum DNA recovery after low temperature storage and high temperature incubations.
- No detectable DNA loss during incubation steps and sample transfer in NGS sample prep and library construction. Ideally suited for sensitive applications with ultra-low nucleic acid input.
Specifications
| FrameStar 96 Well Skirted PCR Plate | |
|---|---|
| Plate length | 127.76 ± 0.25mm |
| Plate width | 85.48 ± 0.25mm |
| Plate height | 16.10 ± 0.25mm |
| Well depth | 15.10 ± 0.10mm |
| Well diameter | 5.50 ± 0.10mm |
| Total well capacity | 0.2ml (200µl) |
| Working volume | <0.1ml (100µl) |
| Distance to center of A1 from top edge | 11.24 ± 0.25mm |
| Distance to center of A1 from left edge | 14.38 ± 0.25mm |
| Pitch (distance between A1 and A2) | 9.00mm |
Compatibility
AGILENT/STRATAGENE
- Thermal Cyclers (96 well block): Robocycler Gradient
- qPCR Cyclers (96 well block): AriaMx
ANALYTIK JENA/BIOMETRA
- Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient/XL | Trobot 96 | SpeedCycler2 (SP, SPR)
BIO-RAD
- Thermal Cyclers (96 well block): C1000 Touch | S1000
- qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH
- Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
- qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
EPPENDORF
- Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
- qPCR Cyclers (96 well block): MasterCycler™ ep realplex
GE HEALTHCARE/AMERSHAM
- Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
PEQLAB/VWR
- Thermal Cyclers (96 well block): peqSTAR 96X
SENSOQUEST
- Thermal Cyclers (96 well block): Labcycler
TECHNE
- Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
- qPCR Cyclers (96 well block): Quantica
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.
Ordering Information
Part Numbers
Use these part numbers to request a quote, free samples, or to contact an expert::
| FrameStar 96 Well Skirted PCR Plate Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H1; working volume: <100µl, total well capacity: 200µl |
|
|---|---|
| 4ti-0960 | clear wells, purple frame; 50 plates |
| 4ti-0960/B | clear wells, blue frame; 50 plates |
| 4ti-0960/C | clear wells, clear frame; 50 plates; Buy Online* |
| 4ti-0960/G | clear wells, green frame; 50 plates |
| 4ti-0960/R | clear wells, red frame; 50 plates |
| 4ti-0960/X | clear wells, black frame; 50 plates |
| 4ti-0960/W | clear wells, white frame; 50 plates; Buy Online* |
| 4ti-0961 | white wells, black frame; 50 plates |
| 4ti-0961/C | white wells, clear frame; 50 plates |
| 4ti-0966 | black wells, black frame; 50 plates |
| 4ti-OX960C/SBC | 4ti-0960/C for forensic use, ethylene oxide treated and single barcoded; 20 plates |
| 4ti-0960/RIG | 4ti-0960/X with extra rigid skirt; 50 plates |
| 4ti-LB0960/RIG | 4ti-0960/RIG, with low DNA binding properties; 50 plates |
| Lids | |
|---|---|
| 4ti-0287 | FrameStar 96 NGS Lid (4ti-0287), for use with 4ti-0960/RIG; 50 lids |
*Online availability to US only
Resources
Application Notes & Tech Notes
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